A proteomic workflow for characterization of human skin biopsies by using 
pico-second infrared laser (PIRL)

Kiani, P.; Eggert, D.; Bussmann, T.; Reuter, J. H.; Uschold, S.; Hansen, N.-O.; Miller, R. J. D.; Schlüter, H.

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A proteomic workflow for characterization of human skin biopsies by using pico-second infrared laser (PIRL)

MPS-Authors

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Uschold, S.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Hansen, N.-O.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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Miller, R. J. D.
Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society;

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引用

Kiani, P., Eggert, D., Bussmann, T., Reuter, J. H., Uschold, S., Hansen, N.-O., Miller, R. J. D., & Schlüter, H. (2016). A proteomic workflow for characterization of human skin biopsies by using pico-second infrared laser (PIRL). Poster presented at 49. Jahrestagung der Deutschen Gesellschaft für Massenspektrometrie (DGMS), Hamburg.

引用: https://hdl.handle.net/21.11116/0000-0001-DE42-B

要旨

Introduction: Human skin is the largest organ of integumentary system that protect the body from e xternal environment and prevent excessive fluid loss [1]. Scientists are closer than ever to understanding the proteomes of skin [2]. However, the protein compositions in each type of cell layers, especially in epidermis, are not accurately defined. For fu rther characterization of protein composition in layers of human skin we have undertaken a proteomic approach by using an innovative, “one - step” extraction and homogenization method, called DIVE (desorption by impulsive excitation of intramolecular vibrati onal states of water molecules in the cell), induced by irradiating tissue with a pico - second infrared laser (PIRL) [3]. The aim of this study was to test if with DIVE epidermis and dermis can be ablated separately.