A protein-binding assay for phallotoxins using muscle actin

Schäfer, A.; Faulstich, Heinz

doi:10.1016/0003-2697(77)90077-X

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A protein-binding assay for phallotoxins using muscle actin

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Schäfer, A.
Max Planck Institute for Medical Research, Max Planck Society;

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Faulstich, Heinz
Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/000326977790077X/pdf?md5=9d0fb75fae0c2fca82f7d68960a6df46&pid=1-s2.0-000326977790077X-main.pdf
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Citation

Schäfer, A., & Faulstich, H. (1977). A protein-binding assay for phallotoxins using muscle actin. Analytical Biochemistry, 83(2), 720-723. doi:10.1016/0003-2697(77)90077-X.

Cite as: https://hdl.handle.net/21.11116/0000-0002-FFCE-8

Abstract

The high affinity of phallotoxins to filamentous actin (KD = 3.6 × 10−8m) has been used to assay small amounts of phallotoxins. The limit of detection was as low as 160 ng of phallotoxins. This is 50 times lower than the limit of all other procedures known so far. The assay was successfully applied to the analysis of phallotoxins in crude extracts of Amanita pholloides mushrooms.