An RNA transcribed from DNA at the origin of phage fd single strand to 
replicative form conversion

Geider, Klaus; Beck, Ewald; Schaller ,  Heinz

doi:10.1073/pnas.75.2.645

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An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion

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Geider, Klaus
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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Citation

Geider, K., Beck, E., & Schaller, H. (1978). An RNA transcribed from DNA at the origin of phage fd single strand to replicative form conversion. Proceedings of the National Academy of Sciences of the United States of America, 75(2), 645-649. doi:10.1073/pnas.75.2.645.

Cite as: https://hdl.handle.net/21.11116/0000-0003-28F5-C

Abstract

Phage fd DNA complexed with DNA binding protein I was used by Escherichia coli RNA polymerase (nucleoside triphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) to synthesize an RNA at the origin of single strand to double strand replication. The isolated ori-RNA gave a simple fingerprint after nucleolytic digestion and has a length of about 30 nucleotides. The characterization of the oligonucleotides from the nuclease digest and the extension of the ori-RNA with DNA polymerase I and subsequent restriction of the DNA gave its exact localization in the fd genome, and its total sequence was deduced from the known DNA sequence in this region.