An order of magnitude faster DNA-PAINT imaging by optimized sequence design and 
buffer conditions

Schueder, Florian; Stein, Johannes; Stehr, Florian; Auer, Alexander; Sperl, Bianca; Strauss, Maximilian T.; Schwille, Petra; Jungmann, Ralf

doi:10.1038/s41592-019-0584-7

Datensatz

DATENSATZ AKTIONENEXPORT

Zur Ablage hinzufügen

Lokale TagsFreigabegeschichteDetailsÜbersicht

Freigegeben

Zeitschriftenartikel

An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions

MPG-Autoren

/persons/resource/persons197583

Schueder, Florian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229547

Stein, Johannes
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229551

Stehr, Florian
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons209115

Auer, Alexander
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78730

Sperl, Bianca
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons186093

Strauss, Maximilian T.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons15815

Schwille, Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172959

Jungmann, Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

Externe Ressourcen

Es sind keine externen Ressourcen hinterlegt

Volltexte (beschränkter Zugriff)

Für Ihren IP-Bereich sind aktuell keine Volltexte freigegeben.

Volltexte (frei zugänglich)

Es sind keine frei zugänglichen Volltexte in PuRe verfügbar

Ergänzendes Material (frei zugänglich)

Es sind keine frei zugänglichen Ergänzenden Materialien verfügbar

Zitation

Schueder, F., Stein, J., Stehr, F., Auer, A., Sperl, B., Strauss, M. T., et al. (2019). An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions. Nature Methods, 16(11), 1101-1104. doi:10.1038/s41592-019-0584-7.

Zitierlink: https://hdl.handle.net/21.11116/0000-0005-9A30-7

Zusammenfassung

DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome this limitation by designing optimized DNA sequences and buffer conditions. We demonstrate our approach in vitro with DNA origami and in situ using cell samples, and achieve an order of magnitude faster imaging speeds without compromising image quality or spatial resolution. This improvement now makes DNA-PAINT applicable to high-throughput studies.