An order of magnitude faster DNA-PAINT imaging by optimized sequence design and 
buffer conditions

Schueder, Florian; Stein, Johannes; Stehr, Florian; Auer, Alexander; Sperl, Bianca; Strauss, Maximilian T.; Schwille, Petra; Jungmann, Ralf

doi:10.1038/s41592-019-0584-7

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An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions

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Schueder, Florian
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229547

Stein, Johannes
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons229551

Stehr, Florian
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons209115

Auer, Alexander
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons78730

Sperl, Bianca
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons186093

Strauss, Maximilian T.
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons15815

Schwille, Petra
Schwille, Petra / Cellular and Molecular Biophysics, Max Planck Institute of Biochemistry, Max Planck Society;

/persons/resource/persons172959

Jungmann, Ralf
Jungmann, Ralf / Molecular Imaging and Bionanotechnology, Max Planck Institute of Biochemistry, Max Planck Society;

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Citation

Schueder, F., Stein, J., Stehr, F., Auer, A., Sperl, B., Strauss, M. T., et al. (2019). An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions. Nature Methods, 16(11), 1101-1104. doi:10.1038/s41592-019-0584-7.

Cite as: https://hdl.handle.net/21.11116/0000-0005-9A30-7

Abstract

DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome this limitation by designing optimized DNA sequences and buffer conditions. We demonstrate our approach in vitro with DNA origami and in situ using cell samples, and achieve an order of magnitude faster imaging speeds without compromising image quality or spatial resolution. This improvement now makes DNA-PAINT applicable to high-throughput studies.