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In vitro gene expression and detergent-free reconstitution of active proteorhodopsin in lipid vesicles.

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Fracasso,  Giorgio
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Körner,  Yvonne
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Gonzales,  David Thomas
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Tang,  T-Y Dora
Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society;

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Citation

Fracasso, G., Körner, Y., Gonzales, D. T., & Tang, T.-Y.-D. (2019). In vitro gene expression and detergent-free reconstitution of active proteorhodopsin in lipid vesicles. Experimental biology and medicine (Maywood, N.J.), 244(4), 314-322. doi:10.1177/1535370218820290.


Cite as: https://hdl.handle.net/21.11116/0000-0006-7DC8-D
Abstract
Our results offer the potential for straightforward, additive-free, and molecularly simple routes to building complex bioreactors based on in vitro transcription-translation systems and lipid vesicles.