Observation of Mg2+.ATP and uncomplexed ATP in slow exchange by 31P-NMR at high 
magnetic fields

Sontheimer, Gerhard M.; Kuhn, W.; Kalbitzer, Hans Robert

doi:10.1016/0006-291X(86)90402-X

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Observation of Mg2+.ATP and uncomplexed ATP in slow exchange by 31P-NMR at high magnetic fields

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Sontheimer, Gerhard M.
Max Planck Institute for Medical Research, Max Planck Society;

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Kalbitzer, Hans Robert
Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society;

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https://www.sciencedirect.com/science/article/pii/0006291X8690402X/pdf?md5=68aa2d21bdd356bca61b0948e9e3236d&pid=1-s2.0-0006291X8690402X-main.pdf
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Citation

Sontheimer, G. M., Kuhn, W., & Kalbitzer, H. R. (1986). Observation of Mg2+.ATP and uncomplexed ATP in slow exchange by 31P-NMR at high magnetic fields. Biochemical and Biophysical Research Communications, 134(3), 1379-1386. doi:10.1016/0006-291X(86)90402-X.

Cite as: https://hdl.handle.net/21.11116/0000-0006-ADB3-D

Abstract

The 31P-NMR lines of the β-phosphate groups in Mg2+.ATP and in metalfree ATP can be observed separately up to 280 K at 8.5 T and up to 285 K at 11.7 T. At 274 K and 8.5T the β-phosporous resonances are in slow exchange at pH values above pH 5, the γ-phosporous resonances are in slow exchange only near pH 6, but in fast exchange at low and high pH-values. The fast exchange condition holds for the α-phosphorous resonances over the entire pH-range. For Ca2+.ATP and metalfree ATP always fast exchange prevails down to the freezing point of water even at 11.7 T. Based on the separate observation of the 31P-NMR signals of Mg2+.ATP and uncomplexed ATP new experiments are proposed and possible sources of error in ‘in vivo’ NMR studies are discussed.