Precipitation of calcium carbonate inside giant unilamellar vesicles composed 
of fluid-phase lipids

Witt, Hannes; Yandrapalli, Naresh; Sari, Merve; Turco, Laura; Robinson, Tom; Steinem, Claudia

doi:10.1021/acs.langmuir.0c02175

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Precipitation of calcium carbonate inside giant unilamellar vesicles composed of fluid-phase lipids

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Yandrapalli, Naresh
Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Robinson, Tom
Tom Robinson, Theorie & Bio-Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society;

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Citation

Witt, H., Yandrapalli, N., Sari, M., Turco, L., Robinson, T., & Steinem, C. (2020). Precipitation of calcium carbonate inside giant unilamellar vesicles composed of fluid-phase lipids. Langmuir, 36(44), 13244-13250. doi:10.1021/acs.langmuir.0c02175.

Cite as: https://hdl.handle.net/21.11116/0000-0007-5339-C

Abstract

Biomineralization of CaCO₃ commonly involves the formation of amorphous CaCO₃ precursor particles that are produced in a confined space surrounded by a lipid bilayer. While the influence of confinement itself has been investigated with different model systems, the impact of an enclosing continuous lipid bilayer on CaCO₃ formation in a confined space is still poorly understood as appropriate model systems are rare. Here, we present a new versatile method based on droplet-based microfluidics to produce fluid-phase giant unilamellar vesicles (GUVs) in the presence of high CaCl₂ concentrations. These GUVs can be readily investigated by means of confocal laser scanning microscopy in combination with bright-field microscopy, demonstrating that the formed CaCO₃ particles are in conformal contact with the fluid-phase lipid bilayer and thus suggesting a strong interaction between the particle and the membrane. Atomic force microscopy adhesion studies with membrane-coated spheres on different CaCO3 crystals corroborated this notion of a strong interaction between the lipids and CaCO₃.