Low-input Capture-C: A chromosome conformation capture assay to analyze 
chromatin architecture in small numbers of cells

Oudelaar, A. M.; Downes, D. J.; Davies, J. O. J.; Hughes, J. R.

doi:10.21769/BioProtoc.2645

Item

ITEM ACTIONSEXPORT

Add to Basket

Please note that a newer version of this item is available:
https://pure.mpg.de/pubman/item/item_3263254_6

DetailsSummary

Released

Journal Article

Low-input Capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells

MPS-Authors

/persons/resource/persons252091

Oudelaar, A. M.
Lise Meitner Group Genome Organization and Regulation, MPI for Biophysical Chemistry, Max Planck Society;

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Oudelaar, A. M., Downes, D. J., Davies, J. O. J., & Hughes, J. R. (2017). Low-input Capture-C: A chromosome conformation capture assay to analyze chromatin architecture in small numbers of cells. Bio-Protocol, 7(23): e2645. doi:10.21769/BioProtoc.2645.

Cite as: https://hdl.handle.net/21.11116/0000-0007-60F3-A

Abstract

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.