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Purification and two-dimensional crystallization of highly active cytochrome b6f complex from spinach

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Dietrich,  Jens
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Kühlbrandt,  Werner
Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Dietrich, J., & Kühlbrandt, W. (1999). Purification and two-dimensional crystallization of highly active cytochrome b6f complex from spinach. FEBS Letters, 463(1-2), 97-102. doi:10.1016/S0014-5793(99)01609-9.


Cite as: http://hdl.handle.net/21.11116/0000-0007-A2E9-B
Abstract
The purification and two-dimensional crystallization of highly active cytochrome b6f complex from spinach is described. The preparation shows all spectroscopic characteristics of the pure complex. The electron transfer activity of 450±60 electrons per s is the highest in vitro activity reported to date. Using dimethyl sulfoxide (DMSO) as a solvent for the electron donor enhanced the performance and reproducibility of the assay. The high yield and the high activity of the protein make it an ideal candidate for biophysical and structural studies. Preliminary two-dimensional crystallization experiments yielded several different forms of two-dimensional and thin three-dimensional crystals, exhibiting varying degrees of order.