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Journal Article

Photoelectric signals generated by bovine rod outer segment disk membranes attached to a lecithin bilayer

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Bamberg,  Ernst
Transport Proteins Group, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Bauer, P. J., Bamberg, E., & Fahr, A. (1984). Photoelectric signals generated by bovine rod outer segment disk membranes attached to a lecithin bilayer. Biophysical Journal, 46(1), 111-116. doi:10.1016/S0006-3495(84)84003-5.


Cite as: http://hdl.handle.net/21.11116/0000-0007-A632-5
Abstract
Purified bovine rod outer segment disk membranes were attached to a lecithin bilayer membrane. After photoexcitation with a 500-nm flash delivered by a dye laser, a negative photovoltage was observed on the bilayer under normal ionic strengths (100 mM KCl), which had a rise phase of 1-3 ms at 20 degrees C. The photoresponse was obviously due to bleaching of rhodopsin as it decreased for successive flashes of light. It originated most probably during the metarhodopsin-I metarhodopsin-II (meta-I-II) transition of rhodopsin because it was pH dependent at 2 degrees C but not at 20 degrees C. At 10 mM KCl, i.e., under hypotonic conditions, a positive photovoltage with slower kinetics than at high salt was observed. As the disk membranes were merely attached to the bilayer membrane, the photovoltage was apparently due to a light-induced transmembrane potential change in the disk membranes. Possible electrogenic mechanisms underlying the photosignal will be discussed.