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lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies

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Grell,  Ernst
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Lewitzki,  Erwin
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Bremer,  Christoph
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Kramer-Schmitt,  Sabine
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Grell, E., Lewitzki, E., Bremer, C., Kramer-Schmitt, S., Weber, J., & Senior, A. E. (1994). lin-Benzo-ATP and-ADP: Versatile fluorescent probes for spectroscopic and biochemical studies. Journal of Fluorescence, 4(3), 247-250. doi:10.1007/BF01878458.


Cite as: http://hdl.handle.net/21.11116/0000-0007-FCBF-7
Abstract
lin-Benzo-adenine nucleotides can act not only as probes for fluorescence studies but also as structural active site probes for enzymes. To understand the basic properties oflin-benzo-ATP and-ADP, protolysis and Mg2+ and Ca2+, binding are investigated between pH 6.2 and pH 8.5 by spectrophotometric and spectrofluorometric titrations. Based on a reaction model, a set of equilibrium constants is determined which is consistent with all available experimental results. The pK values of the Mg2+ and Ca2+ complex oflin-benzo-ATP in the chosen medium are 4.6 and 4.1, respectively, and those for the corresponding diphosphate are 3.1 and 2.8, respectively. Fluorescence and absorption spectra are reported.