Direct visualization of newly synthesized target proteins in situ

tom Dieck, S.; Kochen, L.; Hanus, C.; Heumuller, M.; Bartnik, I.; Nassim-Assir, B.; Merk, K.; Mosler, T.; Garg, S.; Bunse, S.; Tirrell, D. A.; Schuman, Erin M.

doi:10.1038/nmeth.3319

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Direct visualization of newly synthesized target proteins in situ

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Schuman, Erin M.
Synaptic Plasticity Department, Max Planck Institute for Brain Research, Max Planck Society;

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https://www.ncbi.nlm.nih.gov/pubmed/25775042
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Citation

tom Dieck, S., Kochen, L., Hanus, C., Heumuller, M., Bartnik, I., Nassim-Assir, B., et al. (2015). Direct visualization of newly synthesized target proteins in situ. Nat Methods, 12(5), 411-4. doi:10.1038/nmeth.3319.

Cite as: https://hdl.handle.net/21.11116/0000-0007-EF13-7

Abstract

Protein synthesis is a dynamic process that tunes the cellular proteome in response to internal and external demands. Metabolic labeling approaches identify the general proteomic response but cannot visualize specific newly synthesized proteins within cells. Here we describe a technique that couples noncanonical amino acid tagging or puromycylation with the proximity ligation assay to visualize specific newly synthesized proteins and monitor their origin, redistribution and turnover in situ.