The Na+,K+-ATPase carrying the carboxy-terminal Ca2+/calmodulin binding domain 
of the Ca2+ pump has 2Na+,2K+ stoichiometry and lost charge movement in Na+/Na+ 
exchange

Yoshimura, Shige H.; Vasilets, Larisa A.; Ishii, Toshiaki; Takeyasu, Kunio; Schwarz, Wolfgang

doi:10.1016/s0014-5793(98)00202-6

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The Na⁺,K⁺-ATPase carrying the carboxy-terminal Ca²⁺/calmodulin binding domain of the Ca²⁺ pump has 2Na⁺,2K⁺ stoichiometry and lost charge movement in Na⁺/Na⁺ exchange

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Yoshimura, Shige H.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;
Faculty of Integrated Human Studies, Kyoto University, Kyoto 606-01, Japan;

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Vasilets, Larisa A.
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;
Institute of Chemical Physics, Russian Academy of Sciences, 142432 Chernogolovka, Russia;

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Schwarz, Wolfgang
Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society;

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Citation

Yoshimura, S. H., Vasilets, L. A., Ishii, T., Takeyasu, K., & Schwarz, W. (1998). The Na⁺,K⁺-ATPase carrying the carboxy-terminal Ca²⁺/calmodulin binding domain of the Ca²⁺ pump has 2Na⁺,2K⁺ stoichiometry and lost charge movement in Na⁺/Na⁺ exchange. FEBS Letters, 425(1), 71-74. doi:10.1016/s0014-5793(98)00202-6.

Cite as: https://hdl.handle.net/21.11116/0000-0008-392A-A

Abstract

An altered ion-transport stoichiometry from 3Na⁺,2K⁺ to 2Na⁺,2K⁺ is observed in a chimeric Na⁺,K⁺ATPase, which carries the Ca²⁺/calmodulin binding domain (CBD) of the plasma membrane Ca²⁺-ATPase at its carboxy-terminus [Zhao et al., FEBS Lett. 408 (1997) 271-2751. The ouabain-resistant mutant of this chimera (ORalpha1-CBD) was constructed to further investigate the effect of the CBD on ion-transport properties. The ORalpha1-CBD still shows the 2Na⁺,2K⁺ stoichiometry. The loss of electrogenicity is accompanied by the disappearance of transient charge movements in the Na⁺/Na⁺ exchange mode. We conclude that the binding of the third Na⁺ ion, but not of the two others, in 3Na⁺,2K⁺ transport mode apparently senses the electric field, and that the voltage-dependent Na⁺ binding is likely to be lost in the chimera with CBD.