A critical comparison of cellular and cell-free bioproduction systems

Claassens, Nico J.; Burgener, Simon; Voegeli, Bastian; Erb, Tobias J.; Bar-Even, Arren

doi:10.1016/j.copbio.2019.05.003

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A critical comparison of cellular and cell-free bioproduction systems

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Burgener, Simon
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Voegeli, Bastian
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Erb, Tobias J.
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Bar-Even, Arren
Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society;
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Citation

Claassens, N. J., Burgener, S., Voegeli, B., Erb, T. J., & Bar-Even, A. (2019). A critical comparison of cellular and cell-free bioproduction systems. CURRENT OPINION IN BIOTECHNOLOGY, 60, 221-229. doi:10.1016/j.copbio.2019.05.003.

Cite as: https://hdl.handle.net/21.11116/0000-0008-D494-1

Abstract

Conversion of biological feedstocks into value-added chemicals is mostly
performed via microbial fermentation. An emerging alternative approach
is the use of cell-free systems, consisting of purified enzymes and
cofactors. Unfortunately, the in vivo and in vitro research communities
rarely interact, which leads to oversimplifications and exaggerations
that do not permit fair comparison of the two strategies and impede
synergistic interactions. Here, we provide a comprehensive account for
the advantages and drawbacks associated with each strategy, and further
discuss recent research efforts that aim to breach the limits of
cellular and cell-free production. We also explore emerging hybrid
solutions that integrate the benefits of both worlds and could expand
the boundaries of biosynthesis.