Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic 
parts

Miller, Tarryn; Beneyton, Thomas; Schwander, Thomas; Diehl, Christoph; Girault, Mathias; McLean, Richard; Chotel, Tanguy; Claus, Peter; Cortina, Nina Socorro; Baret, Jean-Christophe; Erb, Tobias J.

doi:10.1126/science.aaz6802

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Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts

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Miller, Tarryn
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254704

Schwander, Thomas
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Diehl, Christoph
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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McLean, Richard
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Claus, Peter
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Core Facility Metabolics and small Molecules Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254199

Cortina, Nina Socorro
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Core Facility Metabolics and small Molecules Mass Spectrometry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

/persons/resource/persons254247

Erb, Tobias J.
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Citation

Miller, T., Beneyton, T., Schwander, T., Diehl, C., Girault, M., McLean, R., et al. (2020). Light-powered CO2 fixation in a chloroplast mimic with natural and synthetic parts. SI, 368(6491), 649-+. doi:10.1126/science.aaz6802.

Cite as: https://hdl.handle.net/21.11116/0000-0008-D618-C

Abstract

Nature integrates complex biosynthetic and energy-converting tasks within compartments such as chloroplasts and mitochondria. Chloroplasts convert light into chemical energy, driving carbon dioxide fixation. We used microfluidics to develop a chloroplast mimic by encapsulating and operating photosynthetic membranes in cell-sized droplets. These droplets can be energized by light to power enzymes or enzyme cascades and analyzed for their catalytic properties in multiplex and real time. We demonstrate how these microdroplets can be programmed and controlled by adjusting internal compositions and by using light as an external trigger. We showcase the capability of our platform by integrating the crotonyl-coenzyme A (CoA)/ethylmalony-CoA/hydroxybutyryl-CoA (CETCH) cycle, a synthetic network for carbon dioxide conversion, to create an artificial photosynthetic system that interfaces the natural and the synthetic biological worlds.