English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT

Released

Journal Article

E-box function in a period gene repressed by light

MPS-Authors
/persons/resource/persons283508

Vallone,  D       
Research Group Zebrafish Chronobiology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons283526

Gondi,  SB
Research Group Zebrafish Chronobiology, Max Planck Institute for Developmental Biology, Max Planck Society;

/persons/resource/persons275723

Foulkes,  NS       
Research Group Zebrafish Chronobiology, Max Planck Institute for Developmental Biology, Max Planck Society;

External Resource
No external resources are shared
Fulltext (restricted access)
There are currently no full texts shared for your IP range.
Fulltext (public)
There are no public fulltexts stored in PuRe
Supplementary Material (public)
There is no public supplementary material available
Citation

Vallone, D., Gondi, S., Whitmore, D., & Foulkes, N. (2004). E-box function in a period gene repressed by light. Proceedings of the National Academy of Sciences of the United States of America, 101(12), 4106-4111.


Cite as: https://hdl.handle.net/21.11116/0000-000B-859B-0
Abstract
In most organisms, light plays a key role in the synchronization of the circadian timing system with the environmental day-night cycle. Light pulses that phase-shift the circadian clock also induce the expression of period (per) genes in vertebrates. Here, we report the cloning of a zebrafish per gene, zfper4, which is remarkable in being repressed by light. We have developed an in vivo luciferase reporter assay for this gene in cells that contain a light-entrainable clock. High-definition bioluminescence traces have enabled us to accurately measure phase-shifting of the clock by light. We have also exploited this model to study how four E-box elements in the zfper4 promoter regulate expression. Mutagenesis reveals that the integrity of these four E-boxes is crucial for maintaining low basal expression together with robust rhythmicity and repression by light. Importantly, in the context of a minimal heterologous promoter, the E-box elements also direct a robust circadian rhythm of expression that is significantly phase-advanced compared with the original zfper4 promoter and lacks the light-repression property. Thus, these results reveal flexibility in the phase and light responsiveness of E-box-directed rhythmic expression, depending on the promoter context.