Distinct genetic code expansion strategies for selenocysteine and pyrrolysine 
are reflected in different aminoacyl-tRNA formation systems

Yuan, Jing; O'Donoghue, Patrick; Ambrogelly, Alex; Gundllapalli, Sarath; Sherrer, R. Lynn; Palioura, Sotiria; Simonovic, Miljan; Soell, Dieter

doi:10.1016/j.febslet.2009.11.005

Item

ITEM ACTIONSEXPORT

Add to Basket

Local TagsRelease HistoryDetailsSummary

Released

Journal Article

Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems

MPS-Authors

There are no MPG-Authors in the publication available

External Resource

No external resources are shared

Fulltext (restricted access)

There are currently no full texts shared for your IP range.

Fulltext (public)

There are no public fulltexts stored in PuRe

Supplementary Material (public)

There is no public supplementary material available

Citation

Yuan, J., O'Donoghue, P., Ambrogelly, A., Gundllapalli, S., Sherrer, R. L., Palioura, S., et al. (2010). Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems. FEBS LETTERS, 584(2), 342-349. doi:10.1016/j.febslet.2009.11.005.

Cite as: https://hdl.handle.net/21.11116/0000-000B-EF1A-C

Abstract

Selenocysteine and pyrrolysine, known as the 21st and 22nd amino acids, are directly inserted into growing polypeptides during translation. Selenocysteine is synthesized via a tRNA-dependent pathway and decodes UGA (opal) codons. The incorporation of selenocysteine requires the concerted action of specific RNA and protein elements. In contrast, pyrrolysine is ligated directly to tRNA(Pyl) and inserted into proteins in response to UAG (amber) codons without the need for complex re-coding machinery. Here we review the latest updates on the structure and mechanisms of molecules involved in Sec-tRNA(Sec) and Pyl-tRNA(Pyl) formation as well as the distribution of the Pyl-decoding trait. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.