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Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR

MPG-Autoren
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Zweckstetter,  Markus
Department of NMR Based Structural Biology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;
Research Group of Protein Structure Determination using NMR, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Zitation

Pantoja, C., Ibáñez de Opakua, A., Cima-Omori, M.-S., & Zweckstetter, M. (2023). Determining the Physico-Chemical Composition of Biomolecular Condensates from Spatially-Resolved NMR. Angewandte Chemie International Edition, 62(17): e202218078. doi:10.1002/anie.202218078.


Zitierlink: https://hdl.handle.net/21.11116/0000-000C-F5AA-0
Zusammenfassung
Liquid-Liquid phase separation has emerged as fundamental process underlying the formation of biomolecular condensates. Insights into the composition and structure of biomolecular condensates is, however, complicated by their molecular complexity and dynamics. Here, we introduce an improved spatially-resolved NMR experiment that enables quantitative analysis of the physico-chemical composition of multi-component biomolecular condensates in equilibrium and label-free. Application of spatially-resolved NMR to condensates formed by the Alzheimer's disease-associated protein Tau demonstrates decreased water content, exclusion of the molecular crowding agent dextran, presence of a specific chemical environment of the small molecule DSS, and ≈150-fold increased concentration of Tau inside the condensate. The results suggest that spatially-resolved NMR can have a major impact in understanding the composition and physical chemistry of biomolecular condensates.