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Optimization and characterization of the synthetic secondary chromosome synVicII in Escherichia coli

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Citation

Messerschmidt, S. J., Schindler, D., Zumkeller, C. M., Kemter, F. S., Schallopp, N., & Waldminghaus, T. (2016). Optimization and characterization of the synthetic secondary chromosome synVicII in Escherichia coli. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 4: 96. doi:10.3389/fbioe.2016.00096.


Cite as: https://hdl.handle.net/21.11116/0000-000D-077F-E
Abstract
Learning by building is one of the core ideas of synthetic biology
research. Consequently, building synthetic chromosomes is the way to
fully understand chromosome characteristics. The last years have seen
exciting synthetic chromosome studies. We had previously introduced the
synthetic secondary chromosome synVicII in Escherichia coli. It is based
on the replication mechanism of the secondary chromosome in Vibrio
cholerae. Here, we present a detailed analysis of its genetic
characteristics and a selection approach to optimize replicon stability.
We probe the origin diversity of secondary chromosomes from Vibrionaceae
by construction of several new respective replicons. Finally, we present
a synVicII version 2.0 with several innovations including its full
compatibility with the popular modular cloning (MoClo) assembly system.