Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii 
involves cross-talk between specialized and primary metabolism

Kavakli, Siyar; Grammbitter, Gina L. C.; Bode, Helge B.

doi:10.1101/2022.10.20.513026

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Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism

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Bode, Helge B.
Chemical Biology, Department of Chemistry, Philipps University Marburg, Marburg, Germany;
Molecular Biotechnology, Department of Biosciences, Goethe University Frankfurt, Frankfurt, Germany, External Organizations;
Senckenberg Gesellschaft für Naturforschung, Frankfurt;
Natural Product Function and Engineering, Department of Natural Products in Organismic Interactions, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1101/2022.10.20.513026
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Citation

Kavakli, S., Grammbitter, G. L. C., & Bode, H. B. (2022). Biosynthesis of the multifunctional isopropylstilbene in Photorhabdus laumondii involves cross-talk between specialized and primary metabolism. bioRxiv: the preprint server for biology, 2022.10.20.513026.

Cite as: https://hdl.handle.net/21.11116/0000-000D-0D46-7

Abstract

Isopropylstilbene (IPS) derived from the entomopathogenic bacterium Photorhabdus represents the only known stilbene which is not produced by a plant stilbene synthase but a bacterial PKS II synthase. While the exclusive cyclization reaction, responsible for the formation of the characteristic iso-branched side-chain of the molecule, was studied in the past, some parts of the biosynthetic route remained elusive. In this study, we revealed the role of StlB that is able to produce CoA-derivatives and demonstrated the elongation of cinnamoyl-CoA with enzymes from the bacterial fatty acid biosynthesis pathway. Thus, we deciphered cross-talk between the enzymes from primary and specialized metabolism. These insights led, for the first time, to the production of IPS in a heterologous host.Competing Interest StatementThe authors have declared no competing interest.