Regularly spaced tyrosines in EBF1 mediate BRG1 recruitment and formation of 
nuclear subdiffractive clusters

Zolotarev, Nikolay; Wang, Yuanting; Du, Manyu; Bayer, Marc; Grosschedl, Anna; Cissé, Ibrahim Ibrahim; Grosschedl, Rudolf

doi:10.1101/gad.350828.123

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Regularly spaced tyrosines in EBF1 mediate BRG1 recruitment and formation of nuclear subdiffractive clusters

MPS-Authors

Zolotarev, Nikolay
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Wang, Yuanting
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Du, Manyu
Department of Biological Physics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Bayer, Marc
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

Grosschedl, Anna
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Cissé, Ibrahim Ibrahim
Department of Biological Physics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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Grosschedl, Rudolf
Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society;

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https://genesdev.cshlp.org/content/early/2024/01/17/gad.350828.123
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Citation

Zolotarev, N., Wang, Y., Du, M., Bayer, M., Grosschedl, A., Cissé, I. I., et al. (2024). Regularly spaced tyrosines in EBF1 mediate BRG1 recruitment and formation of nuclear subdiffractive clusters. Genes and Development. doi:10.1101/gad.350828.123.

Cite as: https://hdl.handle.net/21.11116/0000-000E-4140-0

Abstract

B lineage priming by pioneer transcription factor EBF1 requires the function of an intrinsically disordered region (IDR). Here, we examine the role of regularly spaced tyrosines in the IDR as potential determinants of IDR function and activity of EBF1. We found that four Y > A mutations in EBF1 reduced the formation of condensates in vitro and subdiffractive clusters in vivo. Notably, Y > A mutant EBF1 was inefficient in promoting B cell differentiation and showed impaired chromatin binding, recruitment of BRG1, and activation of specific target genes. Thus, regularly spaced tyrosines in the IDR contribute to the biophysical and functional properties of EBF1.