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Combined RNAi and localization for functionally dissecting long non-coding RNAs

MPG-Autoren
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Chakraborty,  Debojyoti
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Kappei,  Dennis
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Theis,  Mirko
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Nitzsche,  Anja
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Ding,  Li
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Paszkowski-Rogacz,  Maciej
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Surendranath,  Vineeth
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Berger,  Nicolas
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

Saar,  Kathrin
Max Planck Society;

/persons/resource/persons219042

Buchholz,  Frank
Max Planck Institute of Molecular Cell Biology and Genetics, Max Planck Society;

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Zitation

Chakraborty, D., Kappei, D., Theis, M., Nitzsche, A., Ding, L., Paszkowski-Rogacz, M., et al. (2012). Combined RNAi and localization for functionally dissecting long non-coding RNAs. Nature Methods, 9(4), 360-362.


Zitierlink: https://hdl.handle.net/21.11116/0000-0001-07F7-1
Zusammenfassung
Whereas methods to comprehensively study cellular roles of protein-coding genes are available, techniques to systematically investigate long noncoding RNAs (lncRNAs), which have been implicated in diverse biological pathways, are limited. Here we report combined knockdown and localization analysis of noncoding RNAs (c-KLAN) that merges functional characterization and localization approaches to study lncRNAs. Using this technique we identified transcripts that regulate mouse embryonic stem cell identity.