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Journal Article

DNA-PAINT MINFLUX nanoscopy

MPS-Authors
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Ostersehlt,  Lynn-Marie       
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Jans,  Daniel C.       
Research Group of Mitochondrial Structure and Dynamics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Wittek,  Anna       
Research Group of Mitochondrial Structure and Dynamics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Keller-Findeisen,  Jan
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Inamdar,  Kaushik       
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Sahl,  Steffen
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Hell,  Stefan W.       
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Jakobs,  Stefan       
Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society;

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Citation

Ostersehlt, L.-M., Jans, D. C., Wittek, A., Keller-Findeisen, J., Inamdar, K., Sahl, S., et al. (2022). DNA-PAINT MINFLUX nanoscopy. Nature Methods, 19, 1072-1075. doi:10.1038/s41592-022-01577-1.


Cite as: https://hdl.handle.net/21.11116/0000-000B-0BA3-1
Abstract
MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations,
has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching
process for single fluorophore separation, initial MINFLUX
implementations have been limited to two color channels.
Here we show that MINFLUX can be effectively combined with
sequentially multiplexed DNA-based labeling (DNA-PAINT),
expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings
of mitochondria in human cells.