Creating new-to-nature carbon fixation: A guide

Schulz-Mirbach, Helena Anna Maria; Dronsella, Beau; He, Hai; Erb, Tobias J.

doi:10.1016/j.ymben.2023.12.012

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Creating new-to-nature carbon fixation: A guide

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Schulz-Mirbach, Helena Anna Maria
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Dronsella, Beau
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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He, Hai
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Erb, Tobias J.
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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https://doi.org/10.1016/j.ymben.2023.12.012
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Citation

Schulz-Mirbach, H. A. M., Dronsella, B., He, H., & Erb, T. J. (2023). Creating new-to-nature carbon fixation: A guide. Metabolic Engineering, 82, 12-28. doi:10.1016/j.ymben.2023.12.012.

Cite as: https://hdl.handle.net/21.11116/0000-000E-1624-1

Abstract

Synthetic biology aims at designing new biological functions from first principles. These new designs allow to expand the natural solution space and overcome the limitations of naturally evolved systems. One example is synthetic CO2-fixation pathways that promise to provide more efficient ways for the capture and conversion of CO2 than natural pathways, such as the Calvin Benson Bassham (CBB) cycle of photosynthesis. In this review, we provide a practical guideline for the design and realization of such new-to-nature CO2-fixation pathways. We introduce the concept of “synthetic CO2-fixation”, and give a general overview over the enzymology and topology of synthetic pathways, before we derive general principles for their design from their eight naturally evolved analogs. We provide a comprehensive summary of synthetic carbon-assimilation pathways and derive a step-by-step, practical guide from the theoretical design to their practical implementation, before ending with an outlook on new developments in the field.