Datensatz

Zusammenfassung

Microarray technology uses the sequence dependent hybridization
(binding) affinity of surface-bound oligonucleotide strands for the
quantification of complex nucleic acid mixtures. In spite of its huge
potential in life science and medicine, microarray oligonucleotide
hybridization remains far from being understood. Taking advantage of
microarray combinatorial possibilities we show that, although surface
bound, the hybridization affinities of single-base mismatched
oligonucleotides can be derived from first principles using parameters
from bulk.