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Journal Article

Evolution of increased complexity and specificity at the dawn of form I Rubiscos

MPS-Authors
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Schulz,  L.
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Zarzycki,  J.
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;

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Erb,  T. J.
Understanding and Building Metabolism, Department of Biochemistry and Synthetic Metabolism, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Center for Synthetic Microbiology (SYNMIKRO), Philipps University of Marburg, Marburg;

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Hochberg,  G. K. A.       
Max Planck Research Group Evolutionary Biochemistry, Max Planck Institute for Terrestrial Microbiology, Max Planck Society;
Center for Synthetic Microbiology (SYNMIKRO), Philipps University of Marburg, Marburg;
Department of Chemistry, University of Marburg;

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https://doi.org/10.1126/science.abq1416
(Publisher version)

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Citation

Schulz, L., Guo, Z., Zarzycki, J., Steinchen, W., Schuller, J. M., Heimerl, T., et al. (2022). Evolution of increased complexity and specificity at the dawn of form I Rubiscos. Science, 378(6616), 155-160. doi:10.1126/science.abq1416.


Cite as: https://hdl.handle.net/21.11116/0000-000B-452C-7
Abstract
The evolution of ribulose-1,5-bisphosphate carboxylase/oxygenases (Rubiscos) that discriminate strongly between their substrate carbon dioxide and the undesired side substrate dioxygen was an important event for photosynthetic organisms adapting to an oxygenated environment. We use ancestral sequence reconstruction to recapitulate this event. We show that Rubisco increased its specificity and carboxylation efficiency through the gain of an accessory subunit before atmospheric oxygen was present. Using structural and biochemical approaches, we retrace how this subunit was gained and became essential. Our work illuminates the emergence of an adaptation to rising ambient oxygen levels, provides a template for investigating the function of interactions that have remained elusive because of their essentiality, and sheds light on the determinants of specificity in Rubisco.